Plasmid

Part:BBa_K4739015:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-11)


Knockout plasmid for vfr


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 70
    Illegal PstI site found at 1843
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal NheI site found at 3791
    Illegal PstI site found at 70
    Illegal PstI site found at 1843
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal BglII site found at 1827
    Illegal BglII site found at 4348
    Illegal XhoI site found at 16
    Illegal XhoI site found at 823
    Illegal XhoI site found at 1470
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 70
    Illegal PstI site found at 1843
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal PstI site found at 70
    Illegal PstI site found at 1843
    Illegal NgoMIV site found at 216
    Illegal NgoMIV site found at 1232
    Illegal NgoMIV site found at 4684
    Illegal NgoMIV site found at 4694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 547
    Illegal BsaI site found at 1545
    Illegal BsaI.rc site found at 1048
    Illegal SapI.rc site found at 5962


Design Notes

The melting temperature and GC content of KO primer.


Source

The sequence of the homologous arm of exoS is from the genome of P. aeruginosa assembled through Gibson assembly.

References